Difference between revisions of "Part:BBa P1002"
Moritz P95 (Talk | contribs) |
|||
| Line 3: | Line 3: | ||
Ampicillin resistance cassette including promoter and coding sequence. It lacks a terminator. | Ampicillin resistance cassette including promoter and coding sequence. It lacks a terminator. | ||
| + | |||
| + | [http://2017.igem.org/Team:Heidelberg, Team Heidelberg 2017] improved this part by insertion of a mutation, yielding a higher enzyme activity (see: <partinfo>BBa_K2398550</partinfo>). <br> | ||
| + | (<small>--[[User:moritz_p95|moritz_p95]] 22:12, 27.10.2017 (UTC)</small>) | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 22:48, 27 October 2017
ampicillin resistance cassette
Ampicillin resistance cassette including promoter and coding sequence. It lacks a terminator.
[http://2017.igem.org/Team:Heidelberg, Team Heidelberg 2017] improved this part by insertion of a mutation, yielding a higher enzyme activity (see: BBa_K2398550).
(--moritz_p95 22:12, 27.10.2017 (UTC))
Usage and Biology
- Used for modular BioBrick vector construction using BioBrick base vector BBa_I51020.
References
Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656099 GenBank EU496092]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]