Difference between revisions of "Cell-free chassis/Commercial E. coli S30"
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=Commercial ''E. coli'' S30= | =Commercial ''E. coli'' S30= | ||
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| − | + | |[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems <font face=georgia color=#cc0033 size=4>Cell-Free Systems (back to Intro)</font>] || Introduction | |
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| − | | | + | |[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 <font face=georgia color=#000066 size=4>Chassis description</font>] || The Commercial ''E. coli'' S30 extract was purchased from Promega. It was prepared by modifications of the method described by Zubay et al (1980). '''''E. coli'' strain B''' deficient in OmpT endoproteinase and lon protease activity was used. |
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| − | The Commercial ''E. coli'' S30 extract was purchased from Promega. It was prepared by modifications of the method described by Zubay et al (1980). '''''E. coli'' strain B''' deficient in OmpT endoproteinase and lon protease activity was used. | + | |
The simple constitutive gene expression device [https://parts.igem.org/wiki/index.php/Part:BBa_I13522 BBa_I13522] has been used to characterize this cell-free chassis | The simple constitutive gene expression device [https://parts.igem.org/wiki/index.php/Part:BBa_I13522 BBa_I13522] has been used to characterize this cell-free chassis | ||
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==References== | ==References== | ||
Please refer to the [https://promega.de/tbs/tb092/tb092.pdf Promega cell extract user guide] for detailed information about the Commercial ''E. coli'' S30 extract. | Please refer to the [https://promega.de/tbs/tb092/tb092.pdf Promega cell extract user guide] for detailed information about the Commercial ''E. coli'' S30 extract. | ||
Revision as of 14:20, 24 October 2007
Commercial E. coli S30
| Cell-Free Systems (back to Intro) | Introduction |
| Chassis description | The Commercial E. coli S30 extract was purchased from Promega. It was prepared by modifications of the method described by Zubay et al (1980). E. coli strain B deficient in OmpT endoproteinase and lon protease activity was used.
The simple constitutive gene expression device BBa_I13522 has been used to characterize this cell-free chassis |