Difference between revisions of "Part:BBa K2462006"
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<partinfo>BBa_K2462006 short</partinfo> | <partinfo>BBa_K2462006 short</partinfo> | ||
| − | This part is a combination of promoter and RBS. We got the sequence of RBS (BBa_B0030) from its part page , and combined it to the promoter got from the plasmid PHIS1525. This plasmid can be used in Bacillus magaterium for the expression of foreign proteins. Then the whole part has been synthesized by AuGCT. | + | This part is a combination of promoter and RBS. We got the sequence of RBS (BBa_B0030) from its part page, and combined it to the promoter got from the plasmid PHIS1525. This plasmid can be used in Bacillus magaterium for the expression of foreign proteins. Then the whole part has been synthesized by AuGCT. |
| − | Bacillus magaterium is a kind of potential genetic engineering host, which is now frequently used in industry and academic fields. It has lots of advantages over | + | Bacillus magaterium is a kind of potential genetic engineering host, which is now frequently used in industry and academic fields. It has lots of advantages over another host as following: |
1. It is able to yield exceptionally high-level foreign protein. | 1. It is able to yield exceptionally high-level foreign protein. | ||
2. Its heredity is stable | 2. Its heredity is stable | ||
| − | 3.The foreign proteins can be secreted out of cell, in that case, we just need to collect the supernatant rather than lyse the cell by sonication. In a word, it can be easily operated. | + | 3.The foreign proteins can be secreted out of the cell, in that case, we just need to collect the supernatant rather than lyse the cell by sonication. In a word, it can be easily operated. |
| − | 4 | + | 4 Endotoxin free for Bacillus magaterium, which is eligible for biosafety requirement. |
| − | However, there are few iGEM teams using these strain as their host, so we are going to be one of the pioneers and we try to find several types of promoter, RBS and terminator and plan to make combinations between them. Our final purpose is to find the most effective way for protein expression in Bacillus magaterium for further uses. | + | However, there are few iGEM teams using these strain as their host, so we are going to be one of the pioneers and we try to find several types of the promoter, RBS and terminator and plan to make combinations between them. Our final purpose is to find the most effective way for protein expression in Bacillus magaterium for further uses. |
Related parts:Part:BBa K2462004 - parts.igem.org https://parts.igem.org/Part:BBa_K2462004 | Related parts:Part:BBa K2462004 - parts.igem.org https://parts.igem.org/Part:BBa_K2462004 | ||
| − | + | Original Part:BBa B0030 - parts.igem.org https://parts.igem.org/Part:BBa_B0030 | |
Revision as of 15:13, 27 October 2017
Promoter for Bacillus megaterium+RBS
This part is a combination of promoter and RBS. We got the sequence of RBS (BBa_B0030) from its part page, and combined it to the promoter got from the plasmid PHIS1525. This plasmid can be used in Bacillus magaterium for the expression of foreign proteins. Then the whole part has been synthesized by AuGCT. Bacillus magaterium is a kind of potential genetic engineering host, which is now frequently used in industry and academic fields. It has lots of advantages over another host as following: 1. It is able to yield exceptionally high-level foreign protein. 2. Its heredity is stable 3.The foreign proteins can be secreted out of the cell, in that case, we just need to collect the supernatant rather than lyse the cell by sonication. In a word, it can be easily operated. 4 Endotoxin free for Bacillus magaterium, which is eligible for biosafety requirement. However, there are few iGEM teams using these strain as their host, so we are going to be one of the pioneers and we try to find several types of the promoter, RBS and terminator and plan to make combinations between them. Our final purpose is to find the most effective way for protein expression in Bacillus magaterium for further uses.
Related parts:Part:BBa K2462004 - parts.igem.org https://parts.igem.org/Part:BBa_K2462004
Original Part:BBa B0030 - parts.igem.org https://parts.igem.org/Part:BBa_B0030
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]