Difference between revisions of "Part:BBa I739018"

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===Purpose===
 
===Purpose===
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<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and defines the learning subsystem.</p>
+
<p>This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and defines the learning subsystem: a multi-inducible toggle switch which can be activated upon addition of LuxR and AHL and reaches steady state through cI or P22 cII. For a detailed describtion, please visit the above mentioned ETHZ project webpage.</p>
  
 
===Testing===
 
===Testing===

Latest revision as of 18:51, 23 October 2007

Learning System (Composite of BBa_I739006 and BBa_I739007)

Part Structure

This composite Biobrick consists of part BBa_I739006 and part BBa_I739007.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and defines the learning subsystem: a multi-inducible toggle switch which can be activated upon addition of LuxR and AHL and reaches steady state through cI or P22 cII. For a detailed describtion, please visit the above mentioned ETHZ project webpage.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1045
    Illegal SapI site found at 1338