Difference between revisions of "Part:BBa I739021"

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===Purpose===
 
===Purpose===
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<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is a composite biobrick including a first generation double promoter. The goal is to show the applicability of such kind of double promoters. In the project description, this part is a composite of the  [https://parts.igem.org/wiki/index.php/Part:BBa_I739014 ''PoC intermediate''] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739005 ''Part 5''] and is cloned into plasmid [https://parts.igem.org/wiki/index.php/Part:BBa_I739201 pBR322BB1]</p>
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<p>This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and is a composite biobrick including a first generation double promoter. The goal is to show the applicability of such kind of double promoters. In the project description, this part is a composite of the  [https://parts.igem.org/wiki/index.php/Part:BBa_I739014 ''PoC intermediate''] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739005 ''Part 5''] and is cloned into plasmid [https://parts.igem.org/wiki/index.php/Part:BBa_I739202 pCK01BB1].</p>
  
 
===Testing===
 
===Testing===

Latest revision as of 10:16, 23 October 2007

Single regulated polycistronic P22 cII +LVA / EYFP +LVA expression cassette

Part Structure

The Biobrick encodes LVA-tagged P22 cII (BBa_C0053) and LVA-tagged EYFP (BBa_E0032) under control of the double promoter BBa_I739101. The ribosome binding sites BBa_B0034 are located upstream of each coding region. The transcription of P22 cII and EYFP is terminated by the double terminator BBa_B0015.

Mode of Action

P22 cII and EYFP production can be repressed by TetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, TetR action is inhibited and the promoter gets derepressed.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and is a composite biobrick including a first generation double promoter. The goal is to show the applicability of such kind of double promoters. In the project description, this part is a composite of the PoC intermediate and Part 5 and is cloned into plasmid pCK01BB1.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 275