Difference between revisions of "Part:BBa I739015"

Revision as of 09:43, 23 October 2007

Double regulated polycistronic P22 cII +LVA / EYFP -LVA expression cassette

Part Structure

The Biobrick encodes LVA-tagged P22 cII (BBa_C0053) and LVA-tagged EYFP (BBa_E0032) under control of the double promoter BBa_I739102 followed by the ribosome binding site BBa_B0034. The transcription of P22 cII and EYFP is terminated by the double terminator BBa_B0015.

Mode of Action

P22 cII and EYFP production can be repressed by cI and/or TetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed. However, there is no inducer available which neutralizes the action of cI.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is a composite of Part 4 and Part 5.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 289

@@ Line 9: / Line 9: @@
 ===Purpose===
-<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is a composite of ''Part 4'' and ''Part 5''. The synthesized P22 cII interacts with ..... </p>
+<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is a composite of [https://parts.igem.org/wiki/index.php/Part:BBa_I739004 ''Part 4''] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739005 ''Part 5''].</p>
 ===Testing===