Difference between revisions of "Part:BBa K2332315"
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|'''RFC standard''' | |'''RFC standard''' | ||
| − | |[https://parts.igem.org/Help:Assembly_standard_10 RFC10], [https://parts.igem.org/Help:Assembly_standard_23 RFC23] & [https://parts.igem.org/Help:Assembly_standard_25 RFC25] compatible | + | |[https://parts.igem.org/Help:Assembly_standard_10 RFC10], [https://parts.igem.org/Help:Assembly_standard_12 RFC12],[https://parts.igem.org/Help:Assembly_standard_23 RFC23] & [https://parts.igem.org/Help:Assembly_standard_25 RFC25] compatible |
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|'''Backbone''' | |'''Backbone''' | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2332315 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2332315 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2332315 parameters</partinfo> | <partinfo>BBa_K2332315 parameters</partinfo> | ||
Revision as of 23:17, 22 October 2017
Plasma membrane delivery system (PMDS, mammalian)
| Plasma Membrane Delivery System (PMDS) | |
|---|---|
| Function | Delivery of protein (e.g. mCherry) to the PM |
| Use in | Mammalian cells |
| Abstraction Hierarchy | Part |
| RFC standard | RFC10, RFC12,RFC23 & RFC25 compatible |
| Backbone | pSB1C3 |
| Submitted by | [http://2017.igem.org/Team:UCL UCL iGEM 2017] |
This coding region translates a transmembrane domain which is destined to go to the plasma membrane via the secretory pathway. While doing so it delivers any 'cytosolic domain insert' that has been cloned into this BioBrick in a plug-and-play via the KpnI-BamHI restriction sites.
Usage and Biology
As stated in the original paper:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2485
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 704
Illegal BsaI.rc site found at 1471
Functional Parameters
Reference
Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Optogenetic control with a photocleavable protein, PhoCl. Nat Methods. 14(4):391-394 (2017) NCBI