Difference between revisions of "Part:BBa I739015"
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===Part Structure===
 
===Part Structure===
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<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged P22 cII ([https://parts.igem.org/wiki/index.php/Part:BBa_C0053 BBa_C0053]) and [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged EYFP ([https://parts.igem.org/wiki/index.php/Part:BBa_E0032 BBa_E0032]) under control of the double promoter [https://parts.igem.org/wiki/index.php/Part:BBa_I739102 BBa_I739102] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. Please notice that there is no terminator in this construct. If you plan to use this construct for double regulated P22 cII production, you should combine it with an appropriate terminator (e.g. [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]).</p>
+
<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged P22 cII ([https://parts.igem.org/wiki/index.php/Part:BBa_C0053 BBa_C0053]) and [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged EYFP ([https://parts.igem.org/wiki/index.php/Part:BBa_E0032 BBa_E0032]) under control of the double promoter [https://parts.igem.org/wiki/index.php/Part:BBa_I739102 BBa_I739102] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of P22 cII and EYFP is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
  
 
===Mode of Action===
 
===Mode of Action===

Revision as of 07:43, 19 October 2007

Double regulated polycistronic P22 cII +LVA / EYFP -LVA expression cassette

Part Structure

The Biobrick encodes LVA-tagged P22 cII (BBa_C0053) and LVA-tagged EYFP (BBa_E0032) under control of the double promoter BBa_I739102 followed by the ribosome binding site BBa_B0034. The transcription of P22 cII and EYFP is terminated by the double terminator BBa_B0015.

Mode of Action

P22 cII and EYFP production can be repressed by cI and/or TetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed. However, there is no inducer available which neutralizes the action of cI.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is a composite of Part 4 and Part 5. The synthesized P22 cII interacts with .....

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 289