Difference between revisions of "Part:BBa K2332313"

Revision as of 18:58, 7 October 2017

E-cadherin (Preproprotein) with CMV promoter & bGH poly(A) tail

This composite part is a device which efficiently encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein.

E-cadherin (Preproprotein) with CMV promoter & bGH poly(A) tail
Function	Cell-Cell Adhesion
Use in	Mammalian cells
RFC standard	RFC10 & RFC21 compatible
Backbone	pSB1C3
Submitted by	[http://2017.igem.org/Team:UCL]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3212
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1414
Illegal BamHI site found at 1490
Illegal BamHI site found at 1606
Illegal BamHI site found at 2530
Illegal BamHI site found at 2832
Illegal XhoI site found at 2214
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 870
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 727
Illegal BsaI site found at 1127
Illegal BsaI site found at 1534
Illegal BsaI site found at 2076
Illegal BsaI.rc site found at 968

@@ Line 6: / Line 6: @@
 {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
-! colspan="2" style="background:#FFBF00;"|CMV:Ha-NLS-dCas9-Linker-VP16-NLS:BGH
+! colspan="2" style="background:#FFBF00;"|E-cadherin (Preproprotein) with CMV promoter & bGH poly(A) tail
 |-
 |'''Function'''
-|gene activation
+|Cell-Cell Adhesion
 |-
 |'''Use in'''
@@ Line 15: / Line 15: @@
 |-
 |'''RFC standard'''
-|[https://parts.igem.org/Help:Assembly_standard_10 RFC10], RFC10 & RFC21 compatible
+|[https://parts.igem.org/Help:Assembly_standard_10 RFC10] & [https://parts.igem.org/Help:Assembly_standard_21 RFC21] compatible
 |-
 |'''Backbone'''