Difference between revisions of "Part:BBa M50048:Design"

 
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===Design Notes===
 
===Design Notes===
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We had to replace the aptamer in the original riboswitch with an aptamer that would target our molecule of interest.
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We replaced the aptamer in the original riboswitch with an aptamer that would target our molecule of interest, atrazine.
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===Source===
 
===Source===

Revision as of 08:23, 12 December 2016


Atrazine riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We replaced the aptamer in the original riboswitch with an aptamer that would target our molecule of interest, atrazine.

Source

Riboswitch components: https://parts.igem.org/Part:BBa_K784005

Atrazine aptamer: Williams, R., Crihfield, C., Gattu, S., Holland, L., & Sooter, L. (2014). In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. IJMS International Journal of Molecular Sciences, 15(8), 14332-14347. doi:10.3390/ijms150814332

References