Difference between revisions of "Part:BBa M50010:Design"

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===Design Notes===
 
===Design Notes===
  
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This promoter is inhibited by a protein encoded by the arsR gene, generating a negative feedback loop.
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This promoter is inhibited by a protein encoded by the arsR gene, generating a negative feedback loop. When arsenic binds to the arsR protein, the promoter is no longer inhibited, allowing transcription to occur. Transcription of the genes following this promoter indicates the presence of arsenic.
  
 
===Source===
 
===Source===

Latest revision as of 03:00, 10 December 2016


Arsenic sensor: ars operon Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter is inhibited by a protein encoded by the arsR gene, generating a negative feedback loop. When arsenic binds to the arsR protein, the promoter is no longer inhibited, allowing transcription to occur. Transcription of the genes following this promoter indicates the presence of arsenic.

Source

This part can be found on E. Coli chromosomal and plasmid DNA. (Escherichia coli K-12 substr. MG1655 arsR. (2016). Ecocyc.org. Retrieved 26 October 2016, from http://ecocyc.org/gene?orgid=ECOLI&id=EG12235#tab=REGULON).

References