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(A time course study for GFP production with this plasmid was also performed over 12 hours. These samples were grown in small volumes (250uL) in a plate reader at 37°. Because these growth conditions were not optimal, GFP was only expressed from the sa...) |
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Latest revision as of 17:41, 5 December 2016
A time course study for GFP production with this plasmid was also performed over 12 hours. These samples were grown in small volumes (250uL) in a plate reader at 37°. Because these growth conditions were not optimal, GFP was only expressed from the sample containing 15mM Arsenate. We found that GFP expression began around 3.5 hours of growth.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 17:41, 5 December 2016 |  | 1,368 × 916 (334 KB) | Maddiehl (Talk | contribs) | We grew JM109 E. Coli cells that had been transformed with our plasmid in 0 to 15 mM KH2AsO4 concentrations for 12 hours to determine the time at which the cells began producing GFP. The total culture volume was 250uL with starting OD600 0.01 of cells... |
| 21:47, 4 December 2016 |  | 1,368 × 916 (334 KB) | Maddiehl (Talk | contribs) | A time course study for GFP production with this plasmid was also performed over 12 hours. These samples were grown in small volumes (250uL) in a plate reader at 37°. Because these growth conditions were not optimal, GFP was only expressed from the sa... |
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