Difference between revisions of "Part:BBa I739005"
Stefan Luzi (Talk | contribs) (→Purpose) |
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===Purpose=== | ===Purpose=== | ||
| − | <p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 1''. .... | + | <p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 1''. ....</p> |
===Testing=== | ===Testing=== | ||
Revision as of 08:54, 12 October 2007
Promoterless expression cassette for EYFP +LVA (B0034.E0032.B0015)
Part Structure
The Biobrick encodes LVA-tagged EYFP (BBa_E0032) downstream of the ribosome binding site BBa_B0034. The transcription of eYFP is terminated by the double terminator BBa_B0015. This construct can be used as second cistron expressing a reporter gene in a multicistronic construct.
Mode of Action
Enhanced yellow fluorescent proteins (EYFP) are fluorochromes, commonly used as reporter proteins.
Purpose
This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 1. ....
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]