Difference between revisions of "Part:BBa K1893000"
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The Las receiver device consists of a constitutively expressed LasR transcription activator [https://parts.igem.org/Part:BBa_C0179 (BBa_C0179)] upstream of a cognate pLas promoter [https://parts.igem.org/Part:BBa_K1893030 (BBa_K1893030)]. LasR is activated in the presence of a 3O-C12-HSL signal and binds to pLas to initiate transcription of a downstream coding sequence. | The Las receiver device consists of a constitutively expressed LasR transcription activator [https://parts.igem.org/Part:BBa_C0179 (BBa_C0179)] upstream of a cognate pLas promoter [https://parts.igem.org/Part:BBa_K1893030 (BBa_K1893030)]. LasR is activated in the presence of a 3O-C12-HSL signal and binds to pLas to initiate transcription of a downstream coding sequence. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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In this case, the constitutively expressed LasR transcriptional regulator [https://parts.igem.org/Part:BBa_C0179 (BBa_C0179)] is activated by the binding of 3O-C12-AHL, an AHL quorum signal. The activated LasR regulator binds to the LasR-inducible promoter, pLas, to initiate transcription of a downstream gene. | In this case, the constitutively expressed LasR transcriptional regulator [https://parts.igem.org/Part:BBa_C0179 (BBa_C0179)] is activated by the binding of 3O-C12-AHL, an AHL quorum signal. The activated LasR regulator binds to the LasR-inducible promoter, pLas, to initiate transcription of a downstream gene. | ||
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===Characterisation=== | ===Characterisation=== | ||
Latest revision as of 03:11, 29 October 2016
Las receiver (LasR+pLas)
The Las receiver device consists of a constitutively expressed LasR transcription activator (BBa_C0179) upstream of a cognate pLas promoter (BBa_K1893030). LasR is activated in the presence of a 3O-C12-HSL signal and binds to pLas to initiate transcription of a downstream coding sequence.
Usage and Biology
The Las receiver is based on the Las quorum-sensing system from Pseudomonas aeruginosa. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
In this case, the constitutively expressed LasR transcriptional regulator (BBa_C0179) is activated by the binding of 3O-C12-AHL, an AHL quorum signal. The activated LasR regulator binds to the LasR-inducible promoter, pLas, to initiate transcription of a downstream gene.
Characterisation
We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here (BBa_K1893001).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
Illegal AgeI site found at 580 - 1000COMPATIBLE WITH RFC[1000]