Difference between revisions of "Part:BBa K1893000"
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<partinfo>BBa_K1893000 short</partinfo>
 
<partinfo>BBa_K1893000 short</partinfo>
  
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The gene encoding the transcriptional activator LasR is downstream a constitutive Anderson promoter, j23101. LasR is activated by 3O-C12-HSL. Included in the construct is the LasR-activated promoter pLas.
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The Las receiver device consists of a constitutively expressed LasR transcription activator [https://parts.igem.org/Part:BBa_C0179 (BBa_C0179)] upstream of a cognate pLas promoter [https://parts.igem.org/Part:BBa_K1893030 (BBa_K1893030)]. LasR is activated in the presence of a 3O-C12-HSL signal and binds to pLas to initiate transcription of a downstream coding sequence.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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The Las receiver is based on the Las quorum-sensing system from <i>Pseudomonas aeruginosa</i>. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
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In this case, the constitutively expressed LasR transcriptional regulator [https://parts.igem.org/Part:BBa_C0179 (BBa_C0179)] is activated by the binding of 3O-C12-AHL, an AHL quorum signal. The activated LasR regulator binds to the LasR-inducible promoter, pLas, to initiate transcription of a downstream gene.
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===Characterisation===
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We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here [https://parts.igem.org/Part:BBa_K1893001 (BBa_K1893001)].
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1893000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1893000 SequenceAndFeatures</partinfo>

Revision as of 03:10, 29 October 2016


Las receiver (LasR+pLas)

The Las receiver device consists of a constitutively expressed LasR transcription activator (BBa_C0179) upstream of a cognate pLas promoter (BBa_K1893030). LasR is activated in the presence of a 3O-C12-HSL signal and binds to pLas to initiate transcription of a downstream coding sequence.

Usage and Biology

The Las receiver is based on the Las quorum-sensing system from Pseudomonas aeruginosa. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.

In this case, the constitutively expressed LasR transcriptional regulator (BBa_C0179) is activated by the binding of 3O-C12-AHL, an AHL quorum signal. The activated LasR regulator binds to the LasR-inducible promoter, pLas, to initiate transcription of a downstream gene.


Characterisation

We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here (BBa_K1893001).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]