Difference between revisions of "Part:BBa K592009"
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This chromoprotein from the coral ''Acropora millepora'', amilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation. | This chromoprotein from the coral ''Acropora millepora'', amilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation. | ||
| − | This part has been adaptated to <i>B. subtilis</i> with a LVA tail. by the UPMC-Paris Team in 2016. (see part BBa K2180019 | + | This part has been adaptated to <i>B. subtilis</i> with a LVA tail. by the UPMC-Paris Team in 2016. (see part BBa K2180019) |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:12, 23 October 2016
amilCP, blue chromoprotein
This chromoprotein from the coral Acropora millepora, amilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation.
This part has been adaptated to B. subtilis with a LVA tail. by the UPMC-Paris Team in 2016. (see part BBa K2180019)
Usage and Biology
This part is useful as a reporter. In the pictures below it's built into BBa_K592015.
iGEM11_Uppsala-Sweden: Expression of chromoproteins. The images above show E coli constitutively expressing amilCP BBa_K592009 (blue), amilGFP BBa_K592010 (yellow) and RFP BBa_E1010 (red). Also see the green chromoprotein cjBlue BBa_K592011.
iGEM12_Uppsala_University: The Uppsala chromoprotein collection and RFP. The image shows pellets of E coli expressing chromoproteins eforRed BBa_K592012, RFP BBa_E1010, cjBlue BBa_K592011, aeBlue BBa_K864401, amilGFP BBa_K592010 and amilCP BBa_K592009.
References
[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.
GenBank: AY646075.1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]