Difference between revisions of "Part:BBa K1362400:Experience"

 
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===Applications of BBa_K1362400===
 
===Applications of BBa_K1362400===
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===Characterization in <i>Nicotiana benthamiana</i>===
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<b>Group</b>: [http://2016.igem.org/Team:Valencia_UPV Valencia_UPV team 2016]
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<b>Author</b>: Valencia UPV iGEM team (Iván Casas Rodrigo, Mónica Victoria Gutiérrez Salazar, Alicia Climent Catalá, Álvaro Ballesteros González)
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<b>Summary</b>
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Valencia UPV 2016 team tested and demonstrated the functionality of inteins in a plant chassis, specifically in <i>Nicotiana benthamiana</i>. We used the inteins with our split-Cas9 ([https://parts.igem.org/Part:BBa_K2017000 BBa_K2017000] and [https://parts.igem.org/Part:BBa_K2017001 BBa_K2017001]). The inteins allowed to fusion of the two parts of the split-Cas9 in vivo, in order to obtain a fully functional Cas9 protein. In Figure 1 it is shown an electrophoresis gel with the results of the testing of split-Cas9 in plants. For further information about the experimental design, check the information of split-Cas9 parts, [https://parts.igem.org/Part:BBa_K2017000 BBa_K2017000] and [https://parts.igem.org/Part:BBa_K2017001 BBa_K2017001]
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[[Image:BBa_K201700_digestionsplitcas9.jpg|600px|thumb|center|'''Figure 1:''' Electrophoresis gel of XT1 amplicons digestion. Mutation efficiencies of Split-intein-Cas9 system and classical Cas9 system are compared by resistant bands signal intensity.]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 14:53, 22 October 2016


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Applications of BBa_K1362400

Characterization in Nicotiana benthamiana

Group: [http://2016.igem.org/Team:Valencia_UPV Valencia_UPV team 2016]

Author: Valencia UPV iGEM team (Iván Casas Rodrigo, Mónica Victoria Gutiérrez Salazar, Alicia Climent Catalá, Álvaro Ballesteros González)

Summary

Valencia UPV 2016 team tested and demonstrated the functionality of inteins in a plant chassis, specifically in Nicotiana benthamiana. We used the inteins with our split-Cas9 (BBa_K2017000 and BBa_K2017001). The inteins allowed to fusion of the two parts of the split-Cas9 in vivo, in order to obtain a fully functional Cas9 protein. In Figure 1 it is shown an electrophoresis gel with the results of the testing of split-Cas9 in plants. For further information about the experimental design, check the information of split-Cas9 parts, BBa_K2017000 and BBa_K2017001

Figure 1: Electrophoresis gel of XT1 amplicons digestion. Mutation efficiencies of Split-intein-Cas9 system and classical Cas9 system are compared by resistant bands signal intensity.

User Reviews

UNIQ596af882006c71e1-partinfo-00000000-QINU UNIQ596af882006c71e1-partinfo-00000001-QINU