Difference between revisions of "Part:BBa K1897028"

Revision as of 15:22, 18 October 2016

p70-33-sfGFP-Terminator

The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 (Part:BBa_K1847008) with a weaker RBS flanked by non-coding sequences (Part:BBa_K1897031), followed by a coding sequence of sfGFP (Part:BBa_K1897033) and a lambda t0 terminator (Part:BBa_K1897030).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 78
Illegal NheI site found at 101
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 589
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 22:02, 14 October 2016 (view source) Keshiniy (Talk \| contribs) ← Older edit		Revision as of 15:22, 18 October 2016 (view source) Phuongnguyen (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K1897028 short</partinfo>		<partinfo>BBa_K1897028 short</partinfo>

−	The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 ([[Part:BBa_K1847008]]) with a weaker RBS flanked by non-coding sequences ([[Part:BBa_K1897031]]), followed by a ~~CDS~~ of sfGFP ([[Part:BBa_K1897033]]) and a lambda t0 terminator ([[Part:BBa_K1897030]]).	+	The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 ([[Part:BBa_K1847008]]) with a weaker RBS flanked by non-coding sequences ([[Part:BBa_K1897031]]), followed by a coding sequence of sfGFP ([[Part:BBa_K1897033]]) and a lambda t0 terminator ([[Part:BBa_K1897030]]).