Difference between revisions of "Part:BBa K1897026"
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<partinfo>BBa_K1897026 short</partinfo>
 
<partinfo>BBa_K1897026 short</partinfo>
  
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The construct contains a high expression promoter J23100 in a cassette ([[Part:BBa_K1897035]]) with a medium RBS containing a Mixed RE Site  ([[Part:BBa_K1897034]]), followed by a coding sequence of ALR ([[Part:BBa_K1172901]]) and a lambda t0 terminator  ([[Part:BBa_K1897030]]).
+
The construct contains a high expression promoter J23100 in a cassette ([[Part:BBa_K1897035]]) with a medium RBS containing a Mixed RE Site  ([[Part:BBa_K1897034]]), followed by a coding sequence of Alanine Racemase ([[Part:BBa_K1172901]]) and a lambda t0 terminator  ([[Part:BBa_K1897030]]).
  
  

Revision as of 14:36, 18 October 2016


J23100-32-ALR-Terminator

The construct contains a high expression promoter J23100 in a cassette (Part:BBa_K1897035) with a medium RBS containing a Mixed RE Site (Part:BBa_K1897034), followed by a coding sequence of Alanine Racemase (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
    Illegal NheI site found at 856
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 780
    Illegal BamHI site found at 1482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal AgeI site found at 898
    Illegal AgeI site found at 1198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 655