Difference between revisions of "Part:BBa B0030"

Revision as of 21:35, 16 October 2016

RBS.1 (strong) -- modified from R. Weiss

Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034.

Usage and Biology

IIT Madras 2016's Characterization

Characterization of popular BioBrick RBSs

Modelling

Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts | modelling]

Experimetnation

Biobrick RBSs | B0030, | B0031, | B0032, | B0034 RBSs were used in our 'Noise in Device' experiment to understand the role of RBS parts in noise.

Note: not compatible with R0053 promoter due to likely transcript secondary structure This combination yielded very low gfp expression (see BBa_I7108). [jcb 8/3/05].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Team Warsaw 2010's measurement

RBS strength (relative to B0034): 91,84%

Functional Parameters

biology	-NA-
efficiency	0.6

(Relative to BBa_B0034)

@@ Line 14: / Line 14: @@
 Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts | modelling]
 == Experimetnation ==
+Biobrick RBSs [https://parts.igem.org/Part:BBa_K1956022 | B0030], [https://parts.igem.org/Part:BBa_K1956023 | B0031], [https://parts.igem.org/Part:BBa_K1956014 | B0032], [https://parts.igem.org/Part:BBa_K1956015 | B0034] RBSs were used in our 'Noise in Device' experiment to understand the role of RBS parts in noise.
 Note: not compatible with R0053 promoter due to likely transcript secondary structure  This combination yielded very low gfp expression (see BBa_I7108).  [jcb 8/3/05].