Difference between revisions of "Part:BBa K1897016:Design"
Shihuiangle (Talk | contribs) |
|||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | There is one HA tag available for characterisation of the LuxR protein produced via western blot. | + | There is one HA tag available for characterisation of the LuxR protein produced via western blot. Note that the stop codon for LuxR <partinfo>BBa_C0062</partinfo> is shifted to after the HA tag. |
− | + | ||
+ | Also, the transcriptional terminators rrnBT1 (from <partinfo>BBa_B0010</partinfo>) + <partinfo>BBa_B0012</partinfo> for LuxR was derived from <partinfo>BBa_B0015</partinfo>, with the first 8 base pairs removed. | ||
===Source=== | ===Source=== | ||
+ | LuxR was obtained from biobrick part <partinfo>BBa_C0062</partinfo>, which was derived from <i>Vibrio fischeri</i>. | ||
+ | The promoter was synthesized based on the sequence obtained from <partinfo>BBa_J23119</partinfo>. | ||
+ | The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. | ||
+ | The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>. | ||
− | + | GFP was obtained from biobrick part <partinfo>BBa_E0040</partinfo>, which was derived from <i>Aequeora victoria</i>. The promoter was synthesized based on the sequence obtained from <partinfo>BBa_R0062</partinfo>. The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>. | |
− | + | ||
===References=== | ===References=== | ||
+ | Mishin, A. S., Subach, F. V., Yampolsky, I. V., King, W., Lukyanov, K. A., & Verkhusha, V. V. (2008). The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore†. Biochemistry, 47(16), 4666-4673. |
Revision as of 20:28, 15 October 2016
LuxR + GFP
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 949
Illegal NheI site found at 972 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 84
Illegal AgeI site found at 992 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 740
Design Notes
There is one HA tag available for characterisation of the LuxR protein produced via western blot. Note that the stop codon for LuxR BBa_C0062 is shifted to after the HA tag.
Also, the transcriptional terminators rrnBT1 (from BBa_B0010) + BBa_B0012 for LuxR was derived from BBa_B0015, with the first 8 base pairs removed.
Source
LuxR was obtained from biobrick part BBa_C0062, which was derived from Vibrio fischeri. The promoter was synthesized based on the sequence obtained from BBa_J23119. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.
GFP was obtained from biobrick part BBa_E0040, which was derived from Aequeora victoria. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.
References
Mishin, A. S., Subach, F. V., Yampolsky, I. V., King, W., Lukyanov, K. A., & Verkhusha, V. V. (2008). The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore†. Biochemistry, 47(16), 4666-4673.