Difference between revisions of "Part:BBa K1992000"
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===Experiments and results=== | ===Experiments and results=== | ||
In order to compare two the RBSs two expression plasmids constructed containing <partinfo>BBa_J23100</partinfo> as promoter, the tested RBS, Tar receptor <partinfo>BBa_K777000</partinfo> and terminator <partinfo>BBa_B0015</partinfo>. The two plasmids <partinfo>BBa_K1992004</partinfo> and <partinfo>BBa_K1992005</partinfo> were cloned to chemoreceptor free E.coli strain (UU1250) and tested by swarming assay as can be seen in figure 1. | In order to compare two the RBSs two expression plasmids constructed containing <partinfo>BBa_J23100</partinfo> as promoter, the tested RBS, Tar receptor <partinfo>BBa_K777000</partinfo> and terminator <partinfo>BBa_B0015</partinfo>. The two plasmids <partinfo>BBa_K1992004</partinfo> and <partinfo>BBa_K1992005</partinfo> were cloned to chemoreceptor free E.coli strain (UU1250) and tested by swarming assay as can be seen in figure 1. | ||
| − | The larger radius of the native RBS suggested higher sensitivity of the chemotaxis system that caused by a higher expression of Tar(1). | + | The larger radius of the native RBS suggested higher a sensitivity of the chemotaxis system that caused by a higher expression of Tar(1). |
[[File:Tar1and2.png|600px|thumb|center|Fig1 (a) K1992004 - Tar expressed w RBS. (b) K1992005 - Tar expressed w the native RBS]] | [[File:Tar1and2.png|600px|thumb|center|Fig1 (a) K1992004 - Tar expressed w RBS. (b) K1992005 - Tar expressed w the native RBS]] | ||
Revision as of 18:05, 15 October 2016
Tar native RBS
Usage and biology
This RBS obtained from the genome of E.coli K12 and located upstream to Tar encoding DNA sequence. The main use of this RBS is to examine its effect on the expression level of Tar chemoreceptor compared to the strongest RBS (B0034) according to the [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas Warsaw 2010's measurement].
Experiments and results
In order to compare two the RBSs two expression plasmids constructed containing BBa_J23100 as promoter, the tested RBS, Tar receptor BBa_K777000 and terminator BBa_B0015. The two plasmids BBa_K1992004 and BBa_K1992005 were cloned to chemoreceptor free E.coli strain (UU1250) and tested by swarming assay as can be seen in figure 1. The larger radius of the native RBS suggested higher a sensitivity of the chemotaxis system that caused by a higher expression of Tar(1).
Reference
1.SOURJIK, Victor; BERG, Howard C. Functional interactions between receptors in bacterial chemotaxis. Nature, 2004, 428.6981: 437-441.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]