Difference between revisions of "Part:BBa K1897014:Design"

 
 
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===Design Notes===
 
===Design Notes===
 
The transcriptional terminators rrnBT1 (from <partinfo>BBa_B0010</partinfo>) + <partinfo>BBa_B0012</partinfo> was derived from <partinfo>BBa_B0015</partinfo>, with the first 8 base pairs removed.
 
The transcriptional terminators rrnBT1 (from <partinfo>BBa_B0010</partinfo>) + <partinfo>BBa_B0012</partinfo> was derived from <partinfo>BBa_B0015</partinfo>, with the first 8 base pairs removed.
−
 
−
 
  
 
===Source===
 
===Source===
  
 
GFP was obtained from biobrick part <partinfo>BBa_E0040</partinfo>, which was derived from <i>Aequeora victoria</i>. The promoter was synthesized based on the sequence obtained from <partinfo>BBa_R0062</partinfo>. The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>.
 
GFP was obtained from biobrick part <partinfo>BBa_E0040</partinfo>, which was derived from <i>Aequeora victoria</i>. The promoter was synthesized based on the sequence obtained from <partinfo>BBa_R0062</partinfo>. The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>.
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===References===
 
===References===
 +
Mishin, A. S., Subach, F. V., Yampolsky, I. V., King, W., Lukyanov, K. A., & Verkhusha, V. V. (2008). The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore†. Biochemistry, 47(16), 4666-4673.

Latest revision as of 14:06, 15 October 2016


GFP with pLuxR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 84
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 740


Design Notes

The transcriptional terminators rrnBT1 (from BBa_B0010) + BBa_B0012 was derived from BBa_B0015, with the first 8 base pairs removed.

Source

GFP was obtained from biobrick part BBa_E0040, which was derived from Aequeora victoria. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.

References

Mishin, A. S., Subach, F. V., Yampolsky, I. V., King, W., Lukyanov, K. A., & Verkhusha, V. V. (2008). The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore†. Biochemistry, 47(16), 4666-4673.