Difference between revisions of "Part:BBa K1897014"

 
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<partinfo>BBa_K1897014 short</partinfo>
 
<partinfo>BBa_K1897014 short</partinfo>
  
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This part was made up of promoter pLuxR <partinfo>BBa_R0062</partinfo>, ribosome binding site (RBS) <partinfo>BBa_B0030</partinfo>, GFP coding sequence <partinfo>BBa_E0040</partinfo> and double terminator <partinfo>BBa_B0015</partinfo>.
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This part was made up of promoter pLuxR <partinfo>BBa_R0062</partinfo>, ribosome binding site (RBS) <partinfo>BBa_B0030</partinfo>, green fluorescent protein (GFP) coding sequence <partinfo>BBa_E0040</partinfo> and double terminator <partinfo>BBa_B0015</partinfo>.
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The GFP protein originated from jellyfish <i>Aequeora victoria</i>, and has a dual-peak absorption-excitation spectrum with a major peak at 396 nm and a smaller peak at 475 nm. Excitation at both peaks results in flurorescence at 503 or 509 nm (Mishin <i>et al.</i>, 2010).
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This part can be used to visually verify that the whole quorum sensing system with LuxR (<partinfo>BBa_K1897008</partinfo>) and LuxI (<partinfo>BBa_K1897008</partinfo>) is working, and that the genes under the control of pLuxR are being expressed.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1897014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1897014 SequenceAndFeatures</partinfo>
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===Construction of composite GFP===
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===Verification of composite GFP===
  
  

Revision as of 13:46, 15 October 2016


GFP with pLuxR

This part was made up of promoter pLuxR BBa_R0062, ribosome binding site (RBS) BBa_B0030, green fluorescent protein (GFP) coding sequence BBa_E0040 and double terminator BBa_B0015.

The GFP protein originated from jellyfish Aequeora victoria, and has a dual-peak absorption-excitation spectrum with a major peak at 396 nm and a smaller peak at 475 nm. Excitation at both peaks results in flurorescence at 503 or 509 nm (Mishin et al., 2010).

Usage and Biology

This part can be used to visually verify that the whole quorum sensing system with LuxR (BBa_K1897008) and LuxI (BBa_K1897008) is working, and that the genes under the control of pLuxR are being expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 84
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 740

Construction of composite GFP

Verification of composite GFP