Difference between revisions of "Part:BBa K1949032"
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<partinfo>BBa_K1949032 short</partinfo> | <partinfo>BBa_K1949032 short</partinfo> | ||
− | This part consists of | + | <span style="margin-left: 10px;">This part consists of P<i>lac</i>(<partinfo>BBa_R0010</partinfo>) + RBS(<partinfo>BBa_B0034</partinfo>) + <i>yafO</i>(<partinfo>BBa_K1949030</partinfo>).<br> |
+ | |||
toxin-antitoxin (TA) systems are cassettes consisting of stable protein, toxin which function as mRNA interferase and unstable protein, antitoxin which destroys function of toxin. Many TA systems exist in the E.coli genome. They can inhibit cell growth or induce cell killing. yafO is one of the toxin and is co-expressed with yafN. | toxin-antitoxin (TA) systems are cassettes consisting of stable protein, toxin which function as mRNA interferase and unstable protein, antitoxin which destroys function of toxin. Many TA systems exist in the E.coli genome. They can inhibit cell growth or induce cell killing. yafO is one of the toxin and is co-expressed with yafN. | ||
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Revision as of 07:01, 12 October 2016
PBAD-rbs-yafO
This part consists of Plac(BBa_R0010) + RBS(BBa_B0034) + yafO(BBa_K1949030).
toxin-antitoxin (TA) systems are cassettes consisting of stable protein, toxin which function as mRNA interferase and unstable protein, antitoxin which destroys function of toxin. Many TA systems exist in the E.coli genome. They can inhibit cell growth or induce cell killing. yafO is one of the toxin and is co-expressed with yafN.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal XhoI site found at 1571 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961