Difference between revisions of "Part:BBa K1897013:Design"

(→‎Source)
(→‎Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Listeriolysin O (LLO) is derived from <i>Listeria monocytogenes</i> and is initially obtained from <partinfo>BBa_K177026</partinfo>. However, after sequencing, the results obtained for sequencing of <partinfo>BBa_K177026</partinfo> did not appear to match that given on the page. We went on to synthesize new LLO (for <partinfo>BBa_K1897013</partinfo>) with the results obtained from sequencing, and also added two HA tags.
+
Listeriolysin O (LLO) is derived from <i>Listeria monocytogenes</i> and is initially obtained from <partinfo>BBa_K177026</partinfo>. However, after sequencing, the results obtained for sequencing of <partinfo>BBa_K177026</partinfo> did not appear to match that given on the page. We went on to synthesize new LLO (for <partinfo>BBa_K1897013</partinfo>) with the results obtained from sequencing, and also added a HA tag.
  
 
===References===
 
===References===

Revision as of 15:15, 11 October 2016


Listeriolysin O


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1482
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LLO CDS BBa_K1897012 is shifted to after the HA tag. Also, the transcriptional terminators rrnBT1 (from BBa_B0010) + BBa_B0012 was derived from BBa_B0015, with the first 8 base pairs removed.


Source

Listeriolysin O (LLO) is derived from Listeria monocytogenes and is initially obtained from BBa_K177026. However, after sequencing, the results obtained for sequencing of BBa_K177026 did not appear to match that given on the page. We went on to synthesize new LLO (for BBa_K1897013) with the results obtained from sequencing, and also added a HA tag.

References