Difference between revisions of "Part:BBa K1949022"

 
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<partinfo>BBa_K1949022 short</partinfo>
 
<partinfo>BBa_K1949022 short</partinfo>
  
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This is acomplete part Plac(<partinfo>BBa_R0010</partinfo>)+RBS(<partinfo>BBa_B0034</partinfo>)+yafN(<partinfo>BBa_K1949020</partinfo>).
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[[Image:yafoyafn.png|thumb|center|400px| Antitoxin YafN inhibit function of toxin YafO]]<br>
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<span style="margin-left: 10px;">This part consists of Plac(<partinfo>BBa_R0010</partinfo>)+RBS(<partinfo>BBa_B0034</partinfo>)+yafN(<partinfo>BBa_K1949020</partinfo>). YafN is an antitoxin corresponding to toxin YafO, and YafN reverses inhabitation of cell growth. yafN and yafO exist as an operon, and YafN also functions as a repressor in the operon. Therefore, yafNO operon’s translation is autoregulated by antitoxin YafN. When Lon protease senses environmental stresses such as DNA damage, the operon becomes active because of degradation of YafN by Lon protease.
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===Characterization===
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<span style="margin-left: 10px;">We did assay experiment and confirmed that YafN reverses the inhibitory effect of YafO on cell growth.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:23, 11 October 2016


Plac-rbs-yafN

Antitoxin YafN inhibit function of toxin YafO

This part consists of Plac(BBa_R0010)+RBS(BBa_B0034)+yafN(BBa_K1949020). YafN is an antitoxin corresponding to toxin YafO, and YafN reverses inhabitation of cell growth. yafN and yafO exist as an operon, and YafN also functions as a repressor in the operon. Therefore, yafNO operon’s translation is autoregulated by antitoxin YafN. When Lon protease senses environmental stresses such as DNA damage, the operon becomes active because of degradation of YafN by Lon protease.

Characterization

We did assay experiment and confirmed that YafN reverses the inhibitory effect of YafO on cell growth.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 304
  • 1000
    COMPATIBLE WITH RFC[1000]