Difference between revisions of "Part:BBa K1897013:Design"

 
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===Design Notes===
 
===Design Notes===
−
There is a HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LLO CDS <partinfo>BBa_K1897012</partinfo> is shifted to after the HA tag.
+
There is a HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LLO CDS <partinfo>BBa_K1897012</partinfo> is shifted to after the HA tag. Also, the transcriptional terminators rrnBT1 (from <partinfo>BBa_B0010</partinfo>) + <partinfo>BBa_B0012</partinfo> was derived from <partinfo>BBa_B0015</partinfo>, with the first 8 base pairs removed.
  
  

Revision as of 19:17, 10 October 2016


Listeriolysin O


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1482
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LLO CDS BBa_K1897012 is shifted to after the HA tag. Also, the transcriptional terminators rrnBT1 (from BBa_B0010) + BBa_B0012 was derived from BBa_B0015, with the first 8 base pairs removed.


Source

Listeriolysin O (LLO) is derived from Listeria monocytogenes and is _______.

References