Difference between revisions of "Part:BBa K1890020"
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This part is based on the part <partinfo>BBa_E0840</partinfo>, which already contains RBS, GFP gene and terminators. By means of PCR we added the strong constitutive promoter <partinfo>BBa_J23100</partinfo>. | This part is based on the part <partinfo>BBa_E0840</partinfo>, which already contains RBS, GFP gene and terminators. By means of PCR we added the strong constitutive promoter <partinfo>BBa_J23100</partinfo>. | ||
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<figure> | <figure> | ||
<img src="https://static.igem.org/mediawiki/2016/4/45/T--TU_Delft--BBa_K1890020_construction.png" width="10%"> | <img src="https://static.igem.org/mediawiki/2016/4/45/T--TU_Delft--BBa_K1890020_construction.png" width="10%"> | ||
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</figcaption> | </figcaption> | ||
</figure> | </figure> | ||
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<h2><span class='h3bb'>Sequence and Features</span></h2> | <h2><span class='h3bb'>Sequence and Features</span></h2> | ||
Revision as of 13:44, 9 October 2016
GFP with strong constitutive promoter, RBS and terminator
Indroduction
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria. This mutant represents a series of mutations resulting in enhanced maturation and emission [1]. It is expressed under control of the strong constitutive promoter BBa_J23100, strong RBS BBa_B0030 and terminators BBa_B0010 and BBa_B0012.
Construction
This part is based on the part BBa_E0840, which already contains RBS, GFP gene and terminators. By means of PCR we added the strong constitutive promoter BBa_J23100.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 700
References
[1] Cormack, B. P., Valdivia, R. H., & Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 173(1), 33-38.