Difference between revisions of "Part:BBa K1982009"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1982009 short</partinfo>
 
<partinfo>BBa_K1982009 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|CRY2
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|-
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|'''Function'''
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|blue light stimulated photoreceptor
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Organism'''
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|''Arabidopsis thaliana''
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|-
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|'''Source'''
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|Arabidopsis thaliana
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|-
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|'''Submitted by'''
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|[http://2016.igem.org/Team:NEU-China NEU-China 2016]
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|}
 
Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS.
 
Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS.
 
Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].
 
Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].

Revision as of 06:16, 9 October 2016


Eukaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)

CRY2
Function blue light stimulated photoreceptor
RFC standard RFC 10
Backbone pSB1C3
Organism Arabidopsis thaliana
Source Arabidopsis thaliana
Submitted by [http://2016.igem.org/Team:NEU-China NEU-China 2016]

Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS. Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 405
    Illegal BglII site found at 864
    Illegal BamHI site found at 1343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 289
    Illegal AgeI site found at 1018
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 641
    Illegal BsaI.rc site found at 50
    Illegal SapI.rc site found at 158