Difference between revisions of "Part:pSB1AT3"

Revision as of 21:49, 10 June 2007

High copy BioBrick assembly plasmid

pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AT3

Plasmid map for pSB1AT3

Usage and Biology

Tetracycline resistant up to 150 ug/ml.

Kanamycin sensitive at 5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3425
Illegal NheI site found at 1270
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3431
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3425
Illegal BamHI site found at 1416
Illegal XhoI site found at 1035
Illegal XhoI site found at 2318
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3425
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3425
Plasmid lacks a suffix.
Illegal XbaI site found at 3440
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1442
Illegal NgoMIV site found at 1810
Illegal NgoMIV site found at 1970
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2464

@@ Line 11: / Line 11: @@
 Kanamycin sensitive at 5 ug/ml
 Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)
+*Primers for these Biobrick vectors can be found in [[Part:BBa_G00100]] (aka VF2) and [[Part:BBa_G00101]] (aka. VR)
 <span class='h3bb'>Sequence and Features</span>