Difference between revisions of "Part:BBa K1890010"
Line 6: Line 6:
 
mCerulean is a cyan fluorescent protein derived from ECFP. It presents a series of mutations that increase its extinction coefficient, quantum yield and fluorescence lifetime [1]. We expressed it under the control of the strong constitutive promoter <partinfo>BBa_J23100</partinfo> and strong RBS <partinfo>BBa_B0030</partinfo>.
 
mCerulean is a cyan fluorescent protein derived from ECFP. It presents a series of mutations that increase its extinction coefficient, quantum yield and fluorescence lifetime [1]. We expressed it under the control of the strong constitutive promoter <partinfo>BBa_J23100</partinfo> and strong RBS <partinfo>BBa_B0030</partinfo>.
  
−
<h2>Sequence and Features</h2>
+
<h2><span class='h3bb'>Sequence and Features</span></h2>
 
<partinfo>BBa_K1890010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1890010 SequenceAndFeatures</partinfo>
  

Revision as of 10:44, 8 October 2016


mCerulean with strong consitutive promoter and RBS

Indroduction

mCerulean is a cyan fluorescent protein derived from ECFP. It presents a series of mutations that increase its extinction coefficient, quantum yield and fluorescence lifetime [1]. We expressed it under the control of the strong constitutive promoter BBa_J23100 and strong RBS BBa_B0030.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Rizzo, M. A., Springer, G. H., Granada, B. & Piston, D. W. An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22, 445–449 (2004).