Difference between revisions of "Part:BBa K1890011"
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<partinfo>BBa_K1890011 short</partinfo>
 
<partinfo>BBa_K1890011 short</partinfo>
  
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<h2>Indroduction</h2>
 
mVenus is a variant of yellow fluorescent protein (YFP) which contains several mutations that make it more stable and provide an improved speed and efficiency of maturation [1]. We expressed it under the control of the strong constitutive promoter <partinfo>BBa_J23100</partinfo> and RBS <partinfo>BBa_B0030</partinfo>.
 
mVenus is a variant of yellow fluorescent protein (YFP) which contains several mutations that make it more stable and provide an improved speed and efficiency of maturation [1]. We expressed it under the control of the strong constitutive promoter <partinfo>BBa_J23100</partinfo> and RBS <partinfo>BBa_B0030</partinfo>.
  
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<h2><span class='h3bb'>Sequence and Features</span></h2>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1890011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1890011 SequenceAndFeatures</partinfo>
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<h2>References</h2>
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[1] Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20, 87–90 (2002).
  
  

Revision as of 10:44, 8 October 2016


mVenus with strong consitutive promoter and RBS

Indroduction

mVenus is a variant of yellow fluorescent protein (YFP) which contains several mutations that make it more stable and provide an improved speed and efficiency of maturation [1]. We expressed it under the control of the strong constitutive promoter BBa_J23100 and RBS BBa_B0030.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20, 87–90 (2002).