Difference between revisions of "Part:BBa K1602051"
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<partinfo>BBa_K1602051 short</partinfo>
 
<partinfo>BBa_K1602051 short</partinfo>
  
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Composite part consisting of an araC-regulated pBAD-promoter (<html><a href="/Part:BBa_K808000">BBa_K808000</a></html>) cloned upstream of RRkey (<html><a href="/Part:BBa_K1602049">BBa_K1602049</a></html>).
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Composite part consisting of an araC-regulated pBAD-promoter (<html><a href="/Part:BBa_K808000">BBa_K808000</a></html>) cloned upstream of RRkey (<html><a href="/Part:BBa_K1602049">BBa_K1602049</a></html>). It is half of a two-part riboregulator-system for <i>E.coli</i> for posttransciptional regulation of gene expression. Upon transcription the produced trans-activating RNA-sequence (taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the formerly masked ribosome binding site (RBS) enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are <html><a href="/Part:BBa_K1602050">RRlocked</a></html> and <html><a href="/Part:BBa_K1602053">RRlocked_site</a></html>.
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<html>
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<center>
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<figure >
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<img width=30%; src="https://static.igem.org/mediawiki/parts/3/3d/Schema_GOI.png">
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<figcaption><b>Figure 1:</b> Interaction of taRNA and crRNA leads to gene expression.</figcaption>
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</figure>
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</center>
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</html>
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Because of the <html><a href="/Part:BBa_K808000">araC-regulated pBAD-promoter</a></html> production of the taRNA is induced by the addition of arabniose. In the presence of glucose araCpBAD shows a very low basal expression level. In combination with the corresponding crRNA-parts (<html><a href="/Part:BBa_K1602050">RRlocked</a></html> and <html><a href="/Part:BBa_K1602053">RRlocked_site</a></html>) this results in an induceable riboregulator-system which represses gene expression in the presence of glucose but is inducable through the addition of arabinose.  
  
 
===Functional Parameters===
 
===Functional Parameters===

Revision as of 05:40, 25 September 2015

araCpBad-RRkey

Composite part consisting of an araC-regulated pBAD-promoter (BBa_K808000) cloned upstream of RRkey (BBa_K1602049). It is half of a two-part riboregulator-system for E.coli for posttransciptional regulation of gene expression. Upon transcription the produced trans-activating RNA-sequence (taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the formerly masked ribosome binding site (RBS) enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are RRlocked and RRlocked_site.

Figure 1: Interaction of taRNA and crRNA leads to gene expression.

Because of the araC-regulated pBAD-promoter production of the taRNA is induced by the addition of arabniose. In the presence of glucose araCpBAD shows a very low basal expression level. In combination with the corresponding crRNA-parts (RRlocked and RRlocked_site) this results in an induceable riboregulator-system which represses gene expression in the presence of glucose but is inducable through the addition of arabinose.

Functional Parameters

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1215
    Illegal SapI site found at 961