Difference between revisions of "Part:BBa K1616005"
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<h3> <font style="color:#b22222">Holin - Endolysin</font> </h3>
 
<h3> <font style="color:#b22222">Holin - Endolysin</font> </h3>
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Holin and endolysin are <b>toxins</b>; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)  
 
Holin and endolysin are <b>toxins</b>; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)  
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<h3> <font style="color:#b22222">Reverse sequence</font> </h3>
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This composite part is composed of the reverse sequence of Holin and the reverse sequence of Endolysin.
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Revision as of 17:32, 21 September 2015

Holin/endolysin reversed - RBS and double T7 terminator

Holin - Endolysin

Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)

Reverse sequence

This composite part is composed of the reverse sequence of Holin and the reverse sequence of Endolysin.





Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 306
    Illegal AgeI site found at 376
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.


Source

BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (double terminator T7 reversed).

References

Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4