Difference between revisions of "Part:BBa K1777003"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1777003 short</partinfo>
 
<partinfo>BBa_K1777003 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|(β-globin SA)-(miR-21 binding site)6-(β-globin SD)
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|-
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|'''Function'''
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|Linear miR-21 sponge
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2015.igem.org/Team:Fudan Fudan 2015]
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|}
  
 
The Fudan iGEM team 2015 designed the part which has 6 miR-21 binding sites and is flanked by β-globing intron1 splicing site. We used it as the linear miR-21 sponge to sequester miR-21, compared with the blank control groups and circRNA sponge groups. In addition, adding cyclizing device to the flanking region of this part, it can be involved into a back-splicing mechanism and become a circRNA sponge.<br>
 
The Fudan iGEM team 2015 designed the part which has 6 miR-21 binding sites and is flanked by β-globing intron1 splicing site. We used it as the linear miR-21 sponge to sequester miR-21, compared with the blank control groups and circRNA sponge groups. In addition, adding cyclizing device to the flanking region of this part, it can be involved into a back-splicing mechanism and become a circRNA sponge.<br>

Revision as of 11:35, 20 September 2015

miR-21 linear sponge

(β-globin SA)-(miR-21 binding site)6-(β-globin SD)
Function Linear miR-21 sponge
Use in Mammalian cells
RFC standard RFC 10
Backbone pSB1C3
Submitted by [http://2015.igem.org/Team:Fudan Fudan 2015]

The Fudan iGEM team 2015 designed the part which has 6 miR-21 binding sites and is flanked by β-globing intron1 splicing site. We used it as the linear miR-21 sponge to sequester miR-21, compared with the blank control groups and circRNA sponge groups. In addition, adding cyclizing device to the flanking region of this part, it can be involved into a back-splicing mechanism and become a circRNA sponge.
As the controls of blank groups, the linear miR-21 sponges have the similiar constructs with "Ouroboros"(Part:BBa_K1777003) and only lack inverted repeat sequences.

Overview of the construct linear miR-21 sponge igem 2015.png

Figure 1: Construct design.

Proof of function

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
    Illegal XhoI site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 423