Difference between revisions of "Part:BBa K1583015"

 
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<partinfo>BBa_K1583015 short</partinfo>
 
<partinfo>BBa_K1583015 short</partinfo>
 
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<htmol>
A linker with the protein sequence GSGGSG was placed in between the CsgA and the affinity tag for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from
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<p>A linker with the protein sequence GSGGSG was placed in between the CsgA (<a href="https://parts.igem.org/Part:BBa_K1583000"target="_blank">BBa_K1583000</a>) and the affinity tags, His and Hydroxyapatite, for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"
engineered curli nanofibres"[1]
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1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features<h3></span>
 
<partinfo>BBa_K1583015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1583015 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K1583015 parameters</partinfo>
 
<partinfo>BBa_K1583015 parameters</partinfo>
 
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<h3>References</h3>
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<p>Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.</p>

Revision as of 01:03, 19 September 2015

Linker in fusion protein <htmol>

A linker with the protein sequence GSGGSG was placed in between the CsgA (<a href="https://parts.igem.org/Part:BBa_K1583000"target="_blank">BBa_K1583000</a>) and the affinity tags, His and Hydroxyapatite, for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"

Sequence and Features<h3></span>

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


<h3>References

<p>Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.</p>