Difference between revisions of "Part:BBa K1850000:Design"

(→‎Design Notes)
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===Source===
 
===Source===
  
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The fimH gene was amplified from the E. coli K12 genome.
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The ''fimH'' gene was amplified from the ''E. coli'' K12 genome.
  
 
===References===
 
===References===

Revision as of 21:24, 18 September 2015


pRha - fimH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected a rhamnose-inducible promoter (BBa_K902065) with a strong ribosome binding site (BBa_B0034), since this promoter is titratable and would allow for controlled expression of the fimH adhesin.

We edited out an illegal PstI cut site in fimH through site-directed mutagenesis.

Source

The fimH gene was amplified from the E. coli K12 genome.

References