Difference between revisions of "Part:BBa K1850000:Design"

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===Design Notes===
 
===Design Notes===
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We selected a rhamnose-inducible promoter  (<partinfo>BBa_K902065</partinfo>)  with a strong ribosome binding site (<partinfo>BBa_B0034</partinfo>), since this promoter is titratable and would allow for controlled expression of the fimH adhesin.
+
We selected a rhamnose-inducible promoter  (<partinfo>BBa_K902065</partinfo>)  with a strong ribosome binding site (<partinfo>BBa_B0034</partinfo>), since this promoter is titratable and would allow for controlled expression of the ''fimH'' adhesin.
  
−
We edited out an illegal PstI cut site in fimH through site-directed mutagenesis.
+
We edited out an illegal PstI cut site in ''fimH'' through site-directed mutagenesis.
  
 
===Source===
 
===Source===

Revision as of 21:24, 18 September 2015


pRha - fimH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected a rhamnose-inducible promoter (BBa_K902065) with a strong ribosome binding site (BBa_B0034), since this promoter is titratable and would allow for controlled expression of the fimH adhesin.

We edited out an illegal PstI cut site in fimH through site-directed mutagenesis.

Source

The fimH gene was amplified from the E. coli K12 genome.

References