Difference between revisions of "Part:BBa K1850000:Design"

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===Design Notes===
 
===Design Notes===
 
We selected a rhamnose-inducible promoter  (<partinfo>BBa_K902065</partinfo>)  with a strong ribosome binding site (<partinfo>BBa_B0034</partinfo>), since this promoter is titratable and would allow for controlled expression of the fimH adhesin.
 
We selected a rhamnose-inducible promoter  (<partinfo>BBa_K902065</partinfo>)  with a strong ribosome binding site (<partinfo>BBa_B0034</partinfo>), since this promoter is titratable and would allow for controlled expression of the fimH adhesin.
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+
We edited out an illegal PstI cut site in fimH through site-directed mutagenesis.
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We edited out an illegal PstI cut site in fimH through site-directed mutagenesis.
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===Source===
 
===Source===

Revision as of 21:19, 18 September 2015


pRha - fimH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected a rhamnose-inducible promoter (BBa_K902065) with a strong ribosome binding site (BBa_B0034), since this promoter is titratable and would allow for controlled expression of the fimH adhesin. We edited out an illegal PstI cut site in fimH through site-directed mutagenesis.

Source

fimH comes from the E. coli K-12 genome, Rhamnose promoter comes from BBa_K902065

References