Difference between revisions of "Part:BBa K1587002"
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<partinfo>BBa_K1587002 short</partinfo> | <partinfo>BBa_K1587002 short</partinfo> | ||
| − | Strong RBS (BBa_B0030). Ferredoxin oxidoreductase (PcyA) from synechocystis (BBa_I15009). Converts biliverdin IXalpha (BV) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes (Figure 1).<br> | + | Strong RBS ([https://parts.igem.org/Part:BBa_B0030 BBa_B0030]). Ferredoxin oxidoreductase (PcyA) from synechocystis ([https://parts.igem.org/Part:BBa_I15009 BBa_I15009]). Converts biliverdin IXalpha (BV) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes (Figure 1).<br> |
The integrity of our construct was checked by sequencing using VF2 primer. | The integrity of our construct was checked by sequencing using VF2 primer. | ||
| + | |||
<center>[[File:TLSE pcyA ho1.jpg]]</center> | <center>[[File:TLSE pcyA ho1.jpg]]</center> | ||
'''Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009)''' | '''Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009)''' | ||
Latest revision as of 20:39, 18 September 2015
pcyA with RBS
Strong RBS (BBa_B0030). Ferredoxin oxidoreductase (PcyA) from synechocystis (BBa_I15009). Converts biliverdin IXalpha (BV) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.
Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009)
pcyA is used to catalyse the formation of phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock. It is usually used in association with ho1 that catalyses the formation of biliverdin (BV) IXα via heme.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 790
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
- 1000COMPATIBLE WITH RFC[1000]