Difference between revisions of "Part:BBa K1638007"
Line 9: Line 9:
 
*'''Emission max''' - 511nm
 
*'''Emission max''' - 511nm
  
−
For further description of the GFP [https://parts.igem.org/wiki/index.php/Part:BBa_E0040 read here].
+
For a more detailed description of this green fluorescent protein, read here: <partinfo>BBa_E0040</partinfo>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:58, 18 September 2015

GFP reporter with flexible linker

This part codes for a green fluorescent protein (GFP) reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression and used in localization assays. A TEV-protease recognition site enables separation of the two proteins. This can be useful in different occasions, e.g. protein purification. This part also contains two transcriptional terminators.

Fluorescence wavelengths

  • Excitation max - 501nm
  • Emission max - 511nm

For a more detailed description of this green fluorescent protein, read here: BBa_E0040

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 674