Difference between revisions of "Part:BBa K1659501"

 
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<partinfo>BBa_K1659501 short</partinfo>
 
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This composite part consists of the AI-2 quorum sensor-inducible promoter [https://parts.igem.org/Part:BBa_K117002 BBa_K117002], a weak ribosome binding site [https://parts.igem.org/Part:BBa_B0031 BBa_B0031], and a GFP coding sequence [https://parts.igem.org/Part:BBa_I746916 BBa_I746916].
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===Usage and Biology===
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===Biology===
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<html><img src="https://static.igem.org/mediawiki/parts/2/23/Oxford15_Lsr_graphic_3.png" width="800" height="456"></html>
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The AI-2 quorum sensing pathway is non-species specific cell-to-cell communication pathway used by both Gram-positive and Gram-negative bacteria and is found in at least 55 strains of them [1][2].
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===References===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1659501 SequenceAndFeatures</partinfo>
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[1] Surette, M.G., Miller, M.B. & Bassler, B.L., 1999. Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production. Proceedings of the National Academy of Sciences of the United States of America, 96(4), pp.1639–1644.
  
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[2] Bassler, B.L., Greenberg, E.P. & Stevens, A.M., 1997. Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. Journal of Bacteriology, 179(12), pp.4043–4045.
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===Functional Parameters===
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<partinfo>BBa_K1659501 parameters</partinfo>
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Revision as of 04:28, 15 September 2015

pLsr + RBS + sfGFP


This composite part consists of the AI-2 quorum sensor-inducible promoter BBa_K117002, a weak ribosome binding site BBa_B0031, and a GFP coding sequence BBa_I746916.


Biology

The AI-2 quorum sensing pathway is non-species specific cell-to-cell communication pathway used by both Gram-positive and Gram-negative bacteria and is found in at least 55 strains of them [1][2].



References

[1] Surette, M.G., Miller, M.B. & Bassler, B.L., 1999. Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production. Proceedings of the National Academy of Sciences of the United States of America, 96(4), pp.1639–1644.

[2] Bassler, B.L., Greenberg, E.P. & Stevens, A.M., 1997. Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. Journal of Bacteriology, 179(12), pp.4043–4045.