Difference between revisions of "Part:BBa K1725080"
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<partinfo>BBa_K1725080 short</partinfo> | <partinfo>BBa_K1725080 short</partinfo> | ||
| − | Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong trasncriptions that can nevertheless be | + | Inverting regulatory region controlled by LacI ([[Part:BBa_C0010]], [Part:BBa_C0012]], etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong trasncriptions that can nevertheless be |
repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) (Lutz and Bujard, 1997) and | repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) (Lutz and Bujard, 1997) and | ||
induced by IPTG in E. Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range | induced by IPTG in E. Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range | ||
Revision as of 21:55, 14 September 2015
Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site
Inverting regulatory region controlled by LacI (Part:BBa_C0010, [Part:BBa_C0012]], etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong trasncriptions that can nevertheless be repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) (Lutz and Bujard, 1997) and induced by IPTG in E. Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range
This part is identical to Part:BBa_R0011 except that an Extra Nhe I site has been added to allow for easy checking for BioBrick insertion using a Nhe I restriction digest
Reference: Lutz R1, Bujard H. Nucleic Acids Res. 1997 25:1203-10. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]