Difference between revisions of "Part:BBa K1725080"
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Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong trasncriptions that can nevertheless be
 
Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong trasncriptions that can nevertheless be
−
repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]) and  
+
repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) (Lutz and Bujard, 1997) and  
 
induced by IPTG in E. Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range
 
induced by IPTG in E. Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range
  
 
This part is identical to [[Part:BBa_R0011]] except that an Extra Nhe I site has been added to allow for easy checking for BioBrick insertion using a Nhe I restriction digest
 
This part is identical to [[Part:BBa_R0011]] except that an Extra Nhe I site has been added to allow for easy checking for BioBrick insertion using a Nhe I restriction digest
 +
 +
Reference:
 +
Lutz R1, Bujard H. Nucleic Acids Res. 1997 25:1203-10. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:54, 14 September 2015

Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site

Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong trasncriptions that can nevertheless be repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) (Lutz and Bujard, 1997) and induced by IPTG in E. Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range

This part is identical to Part:BBa_R0011 except that an Extra Nhe I site has been added to allow for easy checking for BioBrick insertion using a Nhe I restriction digest

Reference: Lutz R1, Bujard H. Nucleic Acids Res. 1997 25:1203-10. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]