Difference between revisions of "Part:BBa K1319016"
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This sequence codes for a TEV protease cleavage site. | This sequence codes for a TEV protease cleavage site. | ||
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| + | ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 03:05, 18 October 2014
TEV protease cleavage site
This sequence codes for a TEV protease cleavage site.
ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.
Usage and Biology
A TEV protease is available codon optimised for E. coli with the part K1319004.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]