Difference between revisions of "Part:BBa K1319014"
AZimmermann (Talk | contribs) |
|||
| Line 2: | Line 2: | ||
<partinfo>BBa_K1319014 short</partinfo> | <partinfo>BBa_K1319014 short</partinfo> | ||
| − | This part expresses a [https://parts.igem.org/Part:BBa_E0040 E0040].[https://parts.igem.org/Part:BBa_K1319002 K1319002] fusion protein ( | + | This part expresses a [https://parts.igem.org/Part:BBa_E0040 E0040].[https://parts.igem.org/Part:BBa_K1319002 K1319002] fusion protein (wtGFP-REACh1) behind a [https://parts.igem.org/Part:BBa_J23101 J23101] promoter. |
| + | [https://parts.igem.org/Part:BBa_K1319002 K1319002] is a '''dark quencher''' that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 02:16, 18 October 2014
Constitutive expression of GFP-REACh2 fusion protein
This part expresses a E0040.K1319002 fusion protein (wtGFP-REACh1) behind a J23101 promoter. K1319002 is a dark quencher that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself.
Usage and Biology
More information about the characterization of this part can be found on the part page of K1319002.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1521
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 706