Difference between revisions of "Part:BBa K1541009"

Revision as of 20:13, 17 October 2014

sfGFP under promoter P(Rhl) with riboregulator RR12

this riboregulated promoter construct contains the quorum sensing promoter BBa_I14017 which can be activated in presence of RhlR (BBa_C0171) and C4-HSL, the product of the enzyme RhlI (BBa_C0170), succeeded by the gene for sfGFP. However, the promoter BBa_I14017 by itself shows some leakiness. Together with the ribogegulator 12^[18] the leakiness could be reduced.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 765
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1541009 short</partinfo>
 [[File:ETH_Zurich_2014_LuxWith_and_WORibo.png|thumb|350px|right]]
+[[File:ETH_Zurich_2014_LuxWith_and_WOSites.png|thumb|350px|right]]
 this riboregulated promoter construct contains the quorum sensing promoter [https://parts.igem.org/Part:BBa_I14017 BBa_I14017] which can be activated in presence of [https://parts.igem.org/Part:BBa_C0171:Experience RhlR (BBa_C0171)] and C4-HSL, the product of the enzyme [https://parts.igem.org/Part:BBa_C0170 RhlI (BBa_C0170)], succeeded by the gene for sfGFP. However, the promoter [https://parts.igem.org/Part:BBa_I14017 BBa_I14017] by itself shows some leakiness. Together with the ribogegulator 12<sup>[[Team:ETH_Zurich/project/references#refCallura|[18]]]</sup> the leakiness could be reduced.