Difference between revisions of "Part:BBa K1442024:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | == Experimentation == |
| + | <p> We compared efficiency of EMCV and NKRF in both HeLa and Huh 7.5 cells. EMCV and HeLa have been previously investigated in HeLa cells however have not been directly compared in Huh 7.5 cells. </p> | ||
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| + | <p><html><body><img src="https://static.igem.org/mediawiki/parts/4/49/Table_IRES.PNG"/></body></html></p> | ||
| + | <p><i>We measured fluorescence using a tecan Magellan plate reader at wavelength 465nm - 530nm (that corresponding to GFP). Each IRES had ten replicates in each cell and hence error bars had an accuracy of 0.1. The fluorescence of media and cells themselves were accounted for in each case and the negative control used was the human optimised forwards GFP <html><body><a href="https://parts.igem.org/Part:BBa_K1442106">part:BBa_K1442106</a></body></html> without IRES included.</i></p> | ||
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Revision as of 13:30, 11 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Experimentation
We compared efficiency of EMCV and NKRF in both HeLa and Huh 7.5 cells. EMCV and HeLa have been previously investigated in HeLa cells however have not been directly compared in Huh 7.5 cells.
We measured fluorescence using a tecan Magellan plate reader at wavelength 465nm - 530nm (that corresponding to GFP). Each IRES had ten replicates in each cell and hence error bars had an accuracy of 0.1. The fluorescence of media and cells themselves were accounted for in each case and the negative control used was the human optimised forwards GFP part:BBa_K1442106 without IRES included.
UNIQa5e1f44ef40cb774-partinfo-00000002-QINU UNIQa5e1f44ef40cb774-partinfo-00000003-QINU