Difference between revisions of "Part:BBa K1379006:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This &sigma;<sup>X</sup> Generator was constructed by restricting-ligating to downstream of [Part:BBa_K880005|BBa_K880005] first and then subsequently to [Part:BBa_B0015|BBa_B0015] in [Part:pSB1C3|pSB1C3].
+
This &sigma;<sup>X</sup> Generator was constructed by restricting-ligating to downstream of [[Part:BBa_K880005|BBa_K880005]] first and then subsequently to [[Part:BBa_B0015|BBa_B0015]] in [[Part:pSB1C3|pSB1C3]].
  
 
===Source===
 
===Source===

Revision as of 15:24, 8 October 2014


S. pneumoniae σx Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 359


Design Notes

This σX Generator was constructed by restricting-ligating to downstream of BBa_K880005 first and then subsequently to BBa_B0015 in pSB1C3.

Source

iGEM 2014 Hong_Kong_HKUST Team has cloned σX from S. pneumoniae strain NCTC7465.

References